Technical Service
Service Introduction
Development of monoclonal cell lines
Vector construction, and the exogenous gene was introduced into CHO/HEK293 cells by transfection, and the resistance gene was screened. The single cell printing system and monoclonal imaging system were combined to screen the monoclonal cell line with stable and high-throughput expression of the target protein.
Inquiry
Service Advantages
1、成熟的HEK293/CHO細(xì)胞表達(dá)平臺(tái)
2、VIPS、Cell Metric 高通量細(xì)胞篩選系統(tǒng)
3、擁有多種細(xì)胞因子、融合蛋白、抗體的細(xì)胞系開(kāi)發(fā)經(jīng)驗(yàn)以及完善的細(xì)胞系開(kāi)發(fā)及上游工藝開(kāi)發(fā)流程
服務(wù)內(nèi)容
| Serial number | Service Content | Deliverables |
| 01 | Cell pool development and evaluation | Stable high-yield engineered cell lines |
| 02 | Cell line development and stability assessment | Monoclonal report |
| 03 | Cell bank establishment | Research cell bank for engineered cell lines |
Project Process
- DNA synthesis and plasmid construction
- Cell pool screening
- Clone screening
- TOP3-6 screening and RCB establishment
- TOP1 screening
Analytical methods
HPLC, SDS-PAGE, Elisa, WB, etc.